Kseniya Rappoport graduated from Saint Petersburg Academy of Theatrical Arts and was immediately invited to join the Maly Drama Theatre. She played Nina Zarechnaya in The Seagull, Elena in Uncle Vania, and Sofia in La doppia ora.
She has appeared in such films and TV series as Streets of Broken Streetlights, Baron, Anna Karenina, Nicholas II (Germany), The Russian Bride, National Security Agent, Empire Under Fire, Calendula Flowers, Prokofiev (Germany), Get Thee From Me, Criminal Petersburg, Homicide, and I Pay Up Front. She starred in the Italian film La sconosciuta (2006) and in the Golden Lion nominated movie La doppia ora (2009), for which she won Volpi Cup (the Best Actress award) at the 66th Venice Film Festival.