Natalia Yevstafieva graduated from the St Petersburg State Rimsky-Korsakov Conservatoire in 1999. From 1997-2000 she was a soloist with the St Petersburg Musorgsky Academic Theatre of Opera and Ballet where she performed as Marina Mnishek (Boris Godunov), Konchakovna (Prince Igor), Olga (Eugene Onegin), Polina (The Queen of Spades), Flora (La traviata), Carmen (Carmen) and Prince Orlovsky (Die Fledermaus).
Soloist with the Mariinsky Academy of Young Singers from 2001, making her Mariinsky Theatre debut the same year as Marfa (Khovanshchina). Natalia Yevstafieva has been a Mariinsky Theatre soloist since 2008.
The singer's concert repertoire includes the mezzo-soprano roles in Beethoven's Ninth Symphony, Verdi's Requiem and arias and romances by Russian, German, Italian and French composers.
Natalia Yevstafieva has toured with the Mariinsky Opera Company to Germany, France and the USA. She has performed as Olga (Eugene Onegin) at Paris' Théâtre du Châtelet and as Carmen (Carmen) at the Coliseu de Lisboa (Portugal).